Virus:Article Title: CRISPR/Cas9-mediated editing of XPA in induced pluripotent stem cells: A model for investigating Xeroderma Pigmentosum and NER dysfunction.
Article Snippet: .. Resource table Unique stem cell line identifier WTSIi018-B-30 Alternative name(s) of stem cell line DU434 Institution IUF Leibniz Institute for Environmental Medicine Contact information of the reported cell line distributor Jean Krutmann; jean.krutmann@IUFduesseldorf.de Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) Age: 55–59 Sex: male Ethnicity: Caucasian; British Cell Source KOLF2.1J, The Jackson Laboratory https://www.jax.org/jax-mice-and-servic es/ipsc Method of reprogramming Sendai Virus Clonality Monoclonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) PCR The cell culture system used iPSCs cultured on geltrex-coated plates and passaged using Accutase Type of the Genetic Modification Patient-specific point mutation Associated disease Xeroderma Pigmentosum (continued on next column) (continued ) Gene/locus XPA homozygous 9q22.33 exon 3 point mutation NM_000380.4(XPA):c.323G>T (p. Cys108Phe) Method of modification / usercustomisable nuclease (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method Plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells PX458 (Addgene #48138) Analysis of the nuclease-targeted allele status Illumina Deep Sequencing and homozygosity confirmed through ONT Sequencing Method of the off-target nuclease activity prediction and surveillance In-silico Descriptive name of the transgene Eukaryotic selective agent resistance cassettes (including inducible, gene/ cell type-specific) (continued on next page) * Corresponding authors at: Environmental Adaptation and Cellular Resilience Laboratory, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (A. Rossi) and Environmentally-Induced Skin Aging Group, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (J. Krutmann). ..
Polymerase Chain Reaction:Article Title: CRISPR/Cas9-mediated editing of XPA in induced pluripotent stem cells: A model for investigating Xeroderma Pigmentosum and NER dysfunction.
Article Snippet: .. Resource table Unique stem cell line identifier WTSIi018-B-30 Alternative name(s) of stem cell line DU434 Institution IUF Leibniz Institute for Environmental Medicine Contact information of the reported cell line distributor Jean Krutmann; jean.krutmann@IUFduesseldorf.de Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) Age: 55–59 Sex: male Ethnicity: Caucasian; British Cell Source KOLF2.1J, The Jackson Laboratory https://www.jax.org/jax-mice-and-servic es/ipsc Method of reprogramming Sendai Virus Clonality Monoclonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) PCR The cell culture system used iPSCs cultured on geltrex-coated plates and passaged using Accutase Type of the Genetic Modification Patient-specific point mutation Associated disease Xeroderma Pigmentosum (continued on next column) (continued ) Gene/locus XPA homozygous 9q22.33 exon 3 point mutation NM_000380.4(XPA):c.323G>T (p. Cys108Phe) Method of modification / usercustomisable nuclease (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method Plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells PX458 (Addgene #48138) Analysis of the nuclease-targeted allele status Illumina Deep Sequencing and homozygosity confirmed through ONT Sequencing Method of the off-target nuclease activity prediction and surveillance In-silico Descriptive name of the transgene Eukaryotic selective agent resistance cassettes (including inducible, gene/ cell type-specific) (continued on next page) * Corresponding authors at: Environmental Adaptation and Cellular Resilience Laboratory, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (A. Rossi) and Environmentally-Induced Skin Aging Group, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (J. Krutmann). ..
Cell Culture:Article Title: CRISPR/Cas9-mediated editing of XPA in induced pluripotent stem cells: A model for investigating Xeroderma Pigmentosum and NER dysfunction.
Article Snippet: .. Resource table Unique stem cell line identifier WTSIi018-B-30 Alternative name(s) of stem cell line DU434 Institution IUF Leibniz Institute for Environmental Medicine Contact information of the reported cell line distributor Jean Krutmann; jean.krutmann@IUFduesseldorf.de Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) Age: 55–59 Sex: male Ethnicity: Caucasian; British Cell Source KOLF2.1J, The Jackson Laboratory https://www.jax.org/jax-mice-and-servic es/ipsc Method of reprogramming Sendai Virus Clonality Monoclonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) PCR The cell culture system used iPSCs cultured on geltrex-coated plates and passaged using Accutase Type of the Genetic Modification Patient-specific point mutation Associated disease Xeroderma Pigmentosum (continued on next column) (continued ) Gene/locus XPA homozygous 9q22.33 exon 3 point mutation NM_000380.4(XPA):c.323G>T (p. Cys108Phe) Method of modification / usercustomisable nuclease (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method Plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells PX458 (Addgene #48138) Analysis of the nuclease-targeted allele status Illumina Deep Sequencing and homozygosity confirmed through ONT Sequencing Method of the off-target nuclease activity prediction and surveillance In-silico Descriptive name of the transgene Eukaryotic selective agent resistance cassettes (including inducible, gene/ cell type-specific) (continued on next page) * Corresponding authors at: Environmental Adaptation and Cellular Resilience Laboratory, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (A. Rossi) and Environmentally-Induced Skin Aging Group, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (J. Krutmann). ..
Modification:Article Title: CRISPR/Cas9-mediated editing of XPA in induced pluripotent stem cells: A model for investigating Xeroderma Pigmentosum and NER dysfunction.
Article Snippet: .. Resource table Unique stem cell line identifier WTSIi018-B-30 Alternative name(s) of stem cell line DU434 Institution IUF Leibniz Institute for Environmental Medicine Contact information of the reported cell line distributor Jean Krutmann; jean.krutmann@IUFduesseldorf.de Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) Age: 55–59 Sex: male Ethnicity: Caucasian; British Cell Source KOLF2.1J, The Jackson Laboratory https://www.jax.org/jax-mice-and-servic es/ipsc Method of reprogramming Sendai Virus Clonality Monoclonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) PCR The cell culture system used iPSCs cultured on geltrex-coated plates and passaged using Accutase Type of the Genetic Modification Patient-specific point mutation Associated disease Xeroderma Pigmentosum (continued on next column) (continued ) Gene/locus XPA homozygous 9q22.33 exon 3 point mutation NM_000380.4(XPA):c.323G>T (p. Cys108Phe) Method of modification / usercustomisable nuclease (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method Plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells PX458 (Addgene #48138) Analysis of the nuclease-targeted allele status Illumina Deep Sequencing and homozygosity confirmed through ONT Sequencing Method of the off-target nuclease activity prediction and surveillance In-silico Descriptive name of the transgene Eukaryotic selective agent resistance cassettes (including inducible, gene/ cell type-specific) (continued on next page) * Corresponding authors at: Environmental Adaptation and Cellular Resilience Laboratory, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (A. Rossi) and Environmentally-Induced Skin Aging Group, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (J. Krutmann). ..
Mutagenesis:Article Title: CRISPR/Cas9-mediated editing of XPA in induced pluripotent stem cells: A model for investigating Xeroderma Pigmentosum and NER dysfunction.
Article Snippet: .. Resource table Unique stem cell line identifier WTSIi018-B-30 Alternative name(s) of stem cell line DU434 Institution IUF Leibniz Institute for Environmental Medicine Contact information of the reported cell line distributor Jean Krutmann; jean.krutmann@IUFduesseldorf.de Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) Age: 55–59 Sex: male Ethnicity: Caucasian; British Cell Source KOLF2.1J, The Jackson Laboratory https://www.jax.org/jax-mice-and-servic es/ipsc Method of reprogramming Sendai Virus Clonality Monoclonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) PCR The cell culture system used iPSCs cultured on geltrex-coated plates and passaged using Accutase Type of the Genetic Modification Patient-specific point mutation Associated disease Xeroderma Pigmentosum (continued on next column) (continued ) Gene/locus XPA homozygous 9q22.33 exon 3 point mutation NM_000380.4(XPA):c.323G>T (p. Cys108Phe) Method of modification / usercustomisable nuclease (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method Plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells PX458 (Addgene #48138) Analysis of the nuclease-targeted allele status Illumina Deep Sequencing and homozygosity confirmed through ONT Sequencing Method of the off-target nuclease activity prediction and surveillance In-silico Descriptive name of the transgene Eukaryotic selective agent resistance cassettes (including inducible, gene/ cell type-specific) (continued on next page) * Corresponding authors at: Environmental Adaptation and Cellular Resilience Laboratory, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (A. Rossi) and Environmentally-Induced Skin Aging Group, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (J. Krutmann). ..
CRISPR:Article Title: CRISPR/Cas9-mediated editing of XPA in induced pluripotent stem cells: A model for investigating Xeroderma Pigmentosum and NER dysfunction.
Article Snippet: .. Resource table Unique stem cell line identifier WTSIi018-B-30 Alternative name(s) of stem cell line DU434 Institution IUF Leibniz Institute for Environmental Medicine Contact information of the reported cell line distributor Jean Krutmann; jean.krutmann@IUFduesseldorf.de Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) Age: 55–59 Sex: male Ethnicity: Caucasian; British Cell Source KOLF2.1J, The Jackson Laboratory https://www.jax.org/jax-mice-and-servic es/ipsc Method of reprogramming Sendai Virus Clonality Monoclonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) PCR The cell culture system used iPSCs cultured on geltrex-coated plates and passaged using Accutase Type of the Genetic Modification Patient-specific point mutation Associated disease Xeroderma Pigmentosum (continued on next column) (continued ) Gene/locus XPA homozygous 9q22.33 exon 3 point mutation NM_000380.4(XPA):c.323G>T (p. Cys108Phe) Method of modification / usercustomisable nuclease (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method Plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells PX458 (Addgene #48138) Analysis of the nuclease-targeted allele status Illumina Deep Sequencing and homozygosity confirmed through ONT Sequencing Method of the off-target nuclease activity prediction and surveillance In-silico Descriptive name of the transgene Eukaryotic selective agent resistance cassettes (including inducible, gene/ cell type-specific) (continued on next page) * Corresponding authors at: Environmental Adaptation and Cellular Resilience Laboratory, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (A. Rossi) and Environmentally-Induced Skin Aging Group, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (J. Krutmann). ..
Plasmid Preparation:Article Title: CRISPR/Cas9-mediated editing of XPA in induced pluripotent stem cells: A model for investigating Xeroderma Pigmentosum and NER dysfunction.
Article Snippet: .. Resource table Unique stem cell line identifier WTSIi018-B-30 Alternative name(s) of stem cell line DU434 Institution IUF Leibniz Institute for Environmental Medicine Contact information of the reported cell line distributor Jean Krutmann; jean.krutmann@IUFduesseldorf.de Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) Age: 55–59 Sex: male Ethnicity: Caucasian; British Cell Source KOLF2.1J, The Jackson Laboratory https://www.jax.org/jax-mice-and-servic es/ipsc Method of reprogramming Sendai Virus Clonality Monoclonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) PCR The cell culture system used iPSCs cultured on geltrex-coated plates and passaged using Accutase Type of the Genetic Modification Patient-specific point mutation Associated disease Xeroderma Pigmentosum (continued on next column) (continued ) Gene/locus XPA homozygous 9q22.33 exon 3 point mutation NM_000380.4(XPA):c.323G>T (p. Cys108Phe) Method of modification / usercustomisable nuclease (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method Plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells PX458 (Addgene #48138) Analysis of the nuclease-targeted allele status Illumina Deep Sequencing and homozygosity confirmed through ONT Sequencing Method of the off-target nuclease activity prediction and surveillance In-silico Descriptive name of the transgene Eukaryotic selective agent resistance cassettes (including inducible, gene/ cell type-specific) (continued on next page) * Corresponding authors at: Environmental Adaptation and Cellular Resilience Laboratory, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (A. Rossi) and Environmentally-Induced Skin Aging Group, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (J. Krutmann). ..
Transfection:Article Title: CRISPR/Cas9-mediated editing of XPA in induced pluripotent stem cells: A model for investigating Xeroderma Pigmentosum and NER dysfunction.
Article Snippet: .. Resource table Unique stem cell line identifier WTSIi018-B-30 Alternative name(s) of stem cell line DU434 Institution IUF Leibniz Institute for Environmental Medicine Contact information of the reported cell line distributor Jean Krutmann; jean.krutmann@IUFduesseldorf.de Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) Age: 55–59 Sex: male Ethnicity: Caucasian; British Cell Source KOLF2.1J, The Jackson Laboratory https://www.jax.org/jax-mice-and-servic es/ipsc Method of reprogramming Sendai Virus Clonality Monoclonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) PCR The cell culture system used iPSCs cultured on geltrex-coated plates and passaged using Accutase Type of the Genetic Modification Patient-specific point mutation Associated disease Xeroderma Pigmentosum (continued on next column) (continued ) Gene/locus XPA homozygous 9q22.33 exon 3 point mutation NM_000380.4(XPA):c.323G>T (p. Cys108Phe) Method of modification / usercustomisable nuclease (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method Plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells PX458 (Addgene #48138) Analysis of the nuclease-targeted allele status Illumina Deep Sequencing and homozygosity confirmed through ONT Sequencing Method of the off-target nuclease activity prediction and surveillance In-silico Descriptive name of the transgene Eukaryotic selective agent resistance cassettes (including inducible, gene/ cell type-specific) (continued on next page) * Corresponding authors at: Environmental Adaptation and Cellular Resilience Laboratory, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (A. Rossi) and Environmentally-Induced Skin Aging Group, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (J. Krutmann). ..
Sequencing:Article Title: CRISPR/Cas9-mediated editing of XPA in induced pluripotent stem cells: A model for investigating Xeroderma Pigmentosum and NER dysfunction.
Article Snippet: .. Resource table Unique stem cell line identifier WTSIi018-B-30 Alternative name(s) of stem cell line DU434 Institution IUF Leibniz Institute for Environmental Medicine Contact information of the reported cell line distributor Jean Krutmann; jean.krutmann@IUFduesseldorf.de Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) Age: 55–59 Sex: male Ethnicity: Caucasian; British Cell Source KOLF2.1J, The Jackson Laboratory https://www.jax.org/jax-mice-and-servic es/ipsc Method of reprogramming Sendai Virus Clonality Monoclonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) PCR The cell culture system used iPSCs cultured on geltrex-coated plates and passaged using Accutase Type of the Genetic Modification Patient-specific point mutation Associated disease Xeroderma Pigmentosum (continued on next column) (continued ) Gene/locus XPA homozygous 9q22.33 exon 3 point mutation NM_000380.4(XPA):c.323G>T (p. Cys108Phe) Method of modification / usercustomisable nuclease (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method Plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells PX458 (Addgene #48138) Analysis of the nuclease-targeted allele status Illumina Deep Sequencing and homozygosity confirmed through ONT Sequencing Method of the off-target nuclease activity prediction and surveillance In-silico Descriptive name of the transgene Eukaryotic selective agent resistance cassettes (including inducible, gene/ cell type-specific) (continued on next page) * Corresponding authors at: Environmental Adaptation and Cellular Resilience Laboratory, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (A. Rossi) and Environmentally-Induced Skin Aging Group, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (J. Krutmann). ..
Activity Assay:Article Title: CRISPR/Cas9-mediated editing of XPA in induced pluripotent stem cells: A model for investigating Xeroderma Pigmentosum and NER dysfunction.
Article Snippet: .. Resource table Unique stem cell line identifier WTSIi018-B-30 Alternative name(s) of stem cell line DU434 Institution IUF Leibniz Institute for Environmental Medicine Contact information of the reported cell line distributor Jean Krutmann; jean.krutmann@IUFduesseldorf.de Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) Age: 55–59 Sex: male Ethnicity: Caucasian; British Cell Source KOLF2.1J, The Jackson Laboratory https://www.jax.org/jax-mice-and-servic es/ipsc Method of reprogramming Sendai Virus Clonality Monoclonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) PCR The cell culture system used iPSCs cultured on geltrex-coated plates and passaged using Accutase Type of the Genetic Modification Patient-specific point mutation Associated disease Xeroderma Pigmentosum (continued on next column) (continued ) Gene/locus XPA homozygous 9q22.33 exon 3 point mutation NM_000380.4(XPA):c.323G>T (p. Cys108Phe) Method of modification / usercustomisable nuclease (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method Plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells PX458 (Addgene #48138) Analysis of the nuclease-targeted allele status Illumina Deep Sequencing and homozygosity confirmed through ONT Sequencing Method of the off-target nuclease activity prediction and surveillance In-silico Descriptive name of the transgene Eukaryotic selective agent resistance cassettes (including inducible, gene/ cell type-specific) (continued on next page) * Corresponding authors at: Environmental Adaptation and Cellular Resilience Laboratory, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (A. Rossi) and Environmentally-Induced Skin Aging Group, IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany (J. Krutmann). ..
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